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KMID : 0364820230590030192
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Korean Journal of Microbiology
2023 Volume.59 No. 3 p.192 ~ p.196
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Comparison of preservation enrichment media for long storage duration of Campylobacter jejuni
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O′la AL-Fawares
Rozan O. Al-Khresieh
Walid Aburayyan
Nesrin Seder
Hala I Al-Daghistani
Nasser El-Banna
Emran M. Abu-Taleb
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Abstract
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Research on Campylobacter jejuni has been greatly impaired due to poor culturing and storage techniques. This study aims to evaluate different preservation methods for C. jejuni for long-term periods of preservation. Three different preservation methods (i, ii, and iii) were tested for their potential for long-term storage of C. jejuni which could be recommended as a simple and reliable method. In the first preservation method (i), 15% glycerol was added to 85% of the bacterial suspension of 4 McFarland standards (McF) and yeast extract. In the second method (ii) the bacterial suspension was supplemented with defibrinated lacked horse blood along with skim milk. Skim milk and 50% glycerol were added to the bacterial suspension in method (iii). Results indicated that method (ii) maintained the highest preservation rate of C. jejuni and the bacteria was capable to grow even after 12 months of storage at -80°C. Method (i) was less efficient than method (ii) where isolates eventually lost their ability to grow after 8 months. Method (iii) showed the least successful preservation rate as many isolates started to die after six months of preservation. This study emphasizes the importance of using defibrinated lacked horse blood along with skim milk during the preservation of bacterial isolates of C. jejuni.
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KEYWORD
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Campylobacter jejuni, defibrinated lacked horse blood, growth recovery, preservation methods, skim milk, ultra-cold mechanical freezers
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